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The attendant told her it had to click twice, or she couldnt ride
Each kidney contains up to a million nephrons
All the stock solutions above were freshly prepared.) was added to equal amounts of protein lysate
Next, membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% (v/v) Tween 20 (TBST) for 1 h at room temperature and probed with primary antibodies against corresponding antigens overnight at 4 C
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